[1]闫 拯,王 乐,袁施彬.响应面法优化提取紫苏籽饼粕迷迭香酸及其抗氧化能力研究[J].西华师范大学学报(自然科学版),2017,38(02):134-140.[doi:DOI:10.16246/j.issn.1673-5072.2017.02.004]
 YAN Zheng,WANG Le,YUAN Shibin.A Study on the Extraction of Rosmarinic Acid from Perilla Seed Meal and Its Antioxidant Capacity[J].Journal of China West Normal University(Natural Sciences),2017,38(02):134-140.[doi:DOI:10.16246/j.issn.1673-5072.2017.02.004]
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响应面法优化提取紫苏籽饼粕迷迭香酸及其抗氧化能力研究()
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《西华师范大学学报(自然科学版)》[ISSN:1673-5072/CN:51-1699/N]

卷:
38
期数:
2017年02期
页码:
134-140
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
A Study on the Extraction of Rosmarinic Acid from Perilla Seed Meal and Its Antioxidant Capacity
作者:
闫 拯王 乐袁施彬
(西华师范大学 a.生命科学学院;b.西南野生动植物资源保护教育部重点实验室,四川 南充637009)
Author(s):
YAN ZhengWANG LeYUAN Shibin
(a.College of Life Science;b.Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University,Nanchong Sichuan 637009,China)
关键词:
紫苏饼粕迷迭香酸响应曲面抗氧化能力
Keywords:
Perillaseed mealrosmarinic acidresponse surface methodologyantioxidant capacity
分类号:
TS202.3
DOI:
DOI:10.16246/j.issn.1673-5072.2017.02.004
文献标志码:
A
摘要:
应用超声波辅助(UAE)提取紫苏饼粕迷迭香酸,通过响应面法(RSM)分析数据,优化提取条件;采用DPPH法、甲基紫法和FRAP法,以抗坏血酸(Vc)为对照,测定迷迭香酸的抗氧化能力。实验结果表明,优化后的响应面提取工艺条件为粒径比60目、料液比1∶10、超声时间71 min及超声温度54 ℃。在此条件下迷迭香酸得率理论值为0.296%,实测值为0.289%;迷迭香酸抗氧化能力弱于Vc,清除自由基能力强于Vc,对铁离子还原能力弱于Vc。其对铁离子还原能力的FRAP值为703.71 mmol Fe2+/100g,清除DPPH和羟基自由基的IC50值为51μg/mL和561 μg/mL。本研究提取方法简单、方便快捷、数据准确,有效提高了紫苏饼粕中迷迭香酸的得率,并且验证紫苏饼粕迷迭香酸具备一定的抗氧化能力,可当做天然抗氧化剂的来源。
Abstract:
Rosmarinic acid is extracted from perilla seed meal with the method of ultrasoundassisted extraction (UAE).The data is analyzed by response surface methodology(RSM) and the extraction conditions are optimized.Its antioxidant ability is determined by DPPH,methyl violet and FRAP,and Vc as control.The results show that the optimum extraction conditions are as follows:particle size 60 mesh,liquid to material ratio 10∶1,ultrasonic time 71 min and ultrasonic temperature 54 ℃.Under these conditions,the theoretical yield of rosmarinic acid is 0.296%,and the measured value is 0.289%;its acid antioxidant capacity is weaker than Vc,scavenging free radicals is stronger than Vc,and iron reduction ability is weaker than Vc.The FRAP value of the reduction ability of ferric ion is 703.71 mmol Fe2+/100g,and the IC50 values of scavenging DPPH and hydroxyl radical are 51 μg/mL and 561 μg/mL respectively.It is concluded that the simplicity,convenience,and accuracy of this extraction method of rosemaric acid help to effectively improve the yield of rosmarinic acid in Perilla Seed Meal.It also proves the possibility of rosmarinic acid from perilla seed meal with certain antioxidant capacity being used as a source of natural antioxidants.

备注/Memo

备注/Memo:
收稿日期:2016-10-17
基金项目:国家自然基金面上项目(31470462)
作者简介:闫拯(1993—),男,北京人,硕士研究生,主要从事大熊猫营养生态研究。
通信作者:袁施彬(1971—),女,四川德阳人,教授,博士后,主要从事野生动物营养生态学研究。E-mail:yshibin@sina.com
更新日期/Last Update: 2017-06-20