[1]谭洪,唐赟,杨艳,等.一株疏水性解烃菌的分离鉴定及特性研究[J].西华师范大学学报(自然科学版),2012,33(02):139-145.[doi:10.16246/j.issn.1673-5072.2012.02.004]
 TAN Hong,TANG Yun,YANG Yan,et al.Study on the Identification and Characteristics of a Strain of Hydrophobic alkanae Degrading Bacteria[J].Journal of China West Normal University(Natural Sciences),2012,33(02):139-145.[doi:10.16246/j.issn.1673-5072.2012.02.004]
点击复制

一株疏水性解烃菌的分离鉴定及特性研究()
分享到:

《西华师范大学学报(自然科学版)》[ISSN:1673-5072/CN:51-1699/N]

卷:
33
期数:
2012年02期
页码:
139-145
栏目:
出版日期:
2012-06-20

文章信息/Info

Title:
Study on the Identification and Characteristics of a Strain of Hydrophobic alkanae Degrading Bacteria
作者:
谭洪;唐赟;杨艳;何春娟;杨牟;
西华师范大学生命科学学院;
Author(s):
TAN HongTANG YunYANG YanHE Chun-juanYANG Mo
( College of Biological Sciences,China West Normal University,Nanchong,637009)
关键词:
石油污染分离鉴定变形斑沙雷氏菌细胞表面疏水性
Keywords:
petroleum pollution isolation and identification serratia proteamaculans cell-surface hydrophobicity
分类号:
Q933
DOI:
10.16246/j.issn.1673-5072.2012.02.004
文献标志码:
A
摘要:
从南充市炼油厂附近被石油污染的土壤中分离获得一株疏水性烷烃降解菌HH1.通过形态学、生理生化特性研究发现菌株HH1为革兰氏阴性,中杆状,周生鞭毛,无荚膜,无芽孢,接触酶阳性,氧化酶阴性,菌体大小0.5-0.8μm×0.9-2.0μm;16S rRNA进化树分析发现HH1与Serratia proteamaculans wg-2和Serratia proteamaculans568的相似性分别为99.6%,菌株HH1归属为变形斑沙雷氏菌(Serratia proteamaculans).同时,采用微生物粘着碳烃化合物法(MATH)对菌株HH1表面疏水性进行研究,结果表明:菌株HH1的表面疏水性为...
Abstract:
A HH1 strain of hydrophobic alkane degrading bacteria HH1 was screened from petroleum-contaminated soils in Nanchong oil refinery. Through morphological,physiological and biochemical analysis,HH1 was catalasepositive, oxidase-negative,Gram-negative,peritrichous,with straight rods,no capsules,no endospores,0. 5 ~ 0. 8um in diameter and 0. 9 ~ 2. 0um in length. And 1316bp of 16S rRNA sequences were amplified from HH1. By blast analysis,16S rRNA showed 99. 6% identity with Serratia proteamaculans wg-2 16S rRNA gene ( accession no. EU627690) and Serratia proteamaculans 568 16S rRNA gene ( accession no. CP000826) . HH1 was finally identified as Serratia proteamaculans. This paper studied on the cell-surface hydrophobicity of HH1 by microorganism adhering to hydrocarbon ( MATH) ,the results showed that the cell-sufrace hydrophobicity of HH1 was 52. 2%,and the cell-sufrace hydrophobicity changed with the variation of incubation time. The n-dodecane degradation was 66. 1% after HH1 was growing on in hydrocarbon degradation medium with 1% ( V/V) of n-docosane at 28℃ and 180r /min for 4d. It had potential in bioremediation of oil contaminated environment.

参考文献/References:


[1] 马强,林爱军,马辉,等. 土壤中中石油烃污染( TPH) 的微生物降解与修复研究进展[J]. 生态毒理学报,2008,3 ( 1) : 1 - 7.
[2] MISHRA V,LAI R,Srinivasan. Enzymes and Operons Mediating Xenobiotic Degradation in Bacteria[J]. Crit Rev Microbio, 2001,27( 2) : 133 - 166.
[3] HARAYAMA S,KASAI Y,HARA A. Microbial Communities in Oil Contaminated Seawater[J]. Environmental Biotechnology, 2004,15: 205 - 214.
[4] ROSENBERG M,GUTNICK D,ROSENBERG E. Adherence of Bacteria to Hydrocarbons: a Simple Method for Measuring Cellsurface Hydrophobicity[J]. FEMS Microbiology Letters,1980,9: 29 - 33.
[5] SUZUKI KAHIRO,MAGUCHI TOYOSHIA,et a1. Immobilization of Prototheca Zopfii in Calcium-algin-ate Baeds for the Degradation of Hydrocarbons[J]. Proces Biochemistry,1998,33( 5) : 541 - 546.
[6] WILLIAM G,WEISBUR G,SUSAN M,et al. 16S Ribosomal DNA Amplification for Phylogenetic Study[J]. Journal of bacteriology, 1991,173( 2) ,697 - 703.
[7] LEGEVEEN R G,HUISMAN G W,PREUSTING H,et al. Formation of Polyesters by Pseudomonas Oleovarans: Effect of Substrates on Formation and Composition of Poly-( R) -3-hydroyalkanoat-es and Poly-( R) -3-hydroxyalkenoates[J]. Applied of Environmental Microbiology. 1988,54( 12) : 2924 - 2932.
[8] NEIDHARDT F C,BLOCH P L,Smith D F. Culture Medium for Enterobacteria[J]. J. Bacterial,1974,119: 736 - 747.
[9] 东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社,2001: 370 - 410.
[10] 中国科学院微生物研究所细菌分类组. 一般细菌常用鉴定方法[M]. 北京: 科学出版社,1978: 114 - 115,125 - 127.
[11] 陈书霞,王晓武,房玉林. 单菌落PCR 法直接快速鉴定重组克隆[J]. 微生物学通报,2006,33( 3) : 52 - 56.
[12] SAITOU N,NEI M. The Neighbor-joining Method: a New Method for Reconstructing Phylogenetic Trees[J]. Mol Biol Evol, 1987,4( 4) : 406 - 425.
[13] ROSENBERG M. Basic and Applied Aspects of Microbial Adhesion at the Hydrocarbon: Water Interface[J]. Crit . Rev. Microbiol, 1991,18( 2) : 159.
[14] HAMADA T,SAMESHIMA Y,HONDA K,et al. A Comparison of Various Methods to Predict Bacterial Predilection for Organic Solvents Used as Reaction Media[J]. Biosci Bioeng,2008,106( 4) : 357 - 362.
[15] AHIMOU F,PAQUOT M,JACQUES P,et al. Influence of Electrical Properties on the Evaluation of the Surface Hydrophobicity of Bacillus Subtilis[J]. Microbiol Methods,2001,45( 2) : 119 - 126.
[16] TAKEUCHI T L,SUZUKI I. Cell Hydrophobicity and Sulfur Adhesion of Thiobacillis Thiooxidans[J]. Applied and Environmental Microbiology,1997,63( 5) : 2058 - 2061.

相似文献/References:

[1]敬 珊,唐 赟.一株降解中长链烷烃菌株TWJ22的解烃特性研究[J].西华师范大学学报(自然科学版),2017,38(02):141.[doi:DOI:10.16246/j.issn.1673-5072.2017.02.005]
 JING Shan,TANG Yun.A Study on The Degradation Characteristics of a Strain of Degrading Mediumlong Chain Alkane[J].Journal of China West Normal University(Natural Sciences),2017,38(02):141.[doi:DOI:10.16246/j.issn.1673-5072.2017.02.005]
[2]冀黎骏,唐赟,谭佳.一株解烃菌TY22的解烃特性研究[J].西华师范大学学报(自然科学版),2015,36(02):135.
 JI Li-jun,TANG Yun,TAN Jia.On the Characterisitics of a Hydrocarbondegradating Strain TY22[J].Journal of China West Normal University(Natural Sciences),2015,36(02):135.

备注/Memo

备注/Memo:

收稿日期: 2011 - 08 - 18
基金项目:四川省教育厅自然科学重点项目( 09ZA123) ,南充市科技局重点项目( 2010SF03)
作者简介:谭洪( 1987 - ) ,男,四川内江人,西华师范大学生命科学学院硕士研究生,主要从事资源微生物学研究.
通讯作者:唐赟( 1970 - ) ,男,四川南充人,西华师范大学生命科学学院副教授,硕士生导生,主要从事资源微生物学研究.
更新日期/Last Update: 2012-06-20