[1]彭聪,徐佳,伍春莲.盐酸石蒜碱对人食管癌细胞Eca-109增殖及凋亡的影响[J].西华师范大学学报(自然科学版),2015,36(01):7-11.
 PENG Cong,XU Jia,WU Chun lian.Effects of Lycorine Hydrochloride on Eca109 Cells via Inhiting Proliferaion and Inducing Apoptosis[J].Journal of China West Normal University(Natural Sciences),2015,36(01):7-11.
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盐酸石蒜碱对人食管癌细胞Eca-109增殖及凋亡的影响
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《西华师范大学学报(自然科学版)》[ISSN:1673-5072/CN:51-1699/N]

卷:
36
期数:
2015年01期
页码:
7-11
栏目:
OA栏目
出版日期:
2015-03-20

文章信息/Info

Title:
Effects of Lycorine Hydrochloride on Eca109 Cells via Inhiting Proliferaion and Inducing Apoptosis
作者:
彭聪徐佳伍春莲
西华师范大学生命科学学院,四川 南充637009
Author(s):
PENG CongXU JiaWU Chunlian
College of Life Science,China West Normal University,Nanchong,637009,China
关键词:
盐酸石蒜碱Eca109细胞细胞活性细胞凋亡
Keywords:
lycorine hydrochloride(LH)Eca109 cellscell viabilitycell apoptosis
分类号:
R285
文献标志码:
B
摘要:
近期研究发现从石蒜科植物体内提取的生物碱,如石蒜碱、盐酸石蒜碱等,具有广泛的抗肿瘤活性功能.本实验通过MTT法测定经不同梯度浓度盐酸石蒜碱(LH)处理后,食管癌Eca109细胞的存活率;通过AO/PI(吖啶橙/碘化丙啶)双染色法观测不同浓度盐酸石蒜碱处理下,食管癌Eca109细胞凋亡的形态;通过流式细胞仪检测Eca109细胞的凋亡率.结果显示,盐酸石蒜碱以时间和剂量依赖性方式抑制食管癌Eca109细胞增殖,晚期凋亡和死亡细胞随盐酸石蒜碱浓度的增大而增多.因此,盐酸石蒜碱能抑制Eca109细胞增殖并诱导其凋亡.
Abstract:
Studies in recent years have shown lycorine or lycorine hydrochloride(LH),a kind of alkaloid extracted from Amaryllidaceae possesses extensive antitumor activities. In this study,we designed MTT assay to measure the survival rate of Eca109 cells treated by lycorine hydrochloride(LH) in gradient concentration.By AO/PI(acridine orange/propidium iodide) double staining,we observed morphological apoptotic characteristics of Eca109 cells treated by lycorine hydrochloride(LH).We carried out analysis of apoptotic cells by flow cytometry.The results showed that LH inhibited Eca109 cells proliferation in a time and dosedependent manner,and the amount of late apoptotic cells and necrotic cells increased with the increasing concentration of LH.Thus LH can inhibit proliferation of Eca109 cells and induce apoptosis.

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备注/Memo

备注/Memo:
收稿日期:2014-09-10
基金项目:四川省教育厅重大培育项目(13CZ0029),三峡库区生态环境与生物资源省部共建重点实验室开放课题基金项目(SKL-2011-05),中国博士后基金(2013M540391)
作者简介:彭聪(1988-),男,湖北省潜江人,西华师范大学硕士研究生,主要从事细胞学与天然抗肿瘤活性成分的研究.
通讯作者:伍春莲(1976-),女,四川彭州人,西华师范大学生命科学学院副教授,博士,主要从事细胞和分子生物学的研究和教学.
更新日期/Last Update: 2015-03-20