[1]徐佳,伍春莲,彭聪,等.雷公藤红素对人肺癌细胞增殖与凋亡的影响[J].西华师范大学学报(自然科学版),2014,35(01):51-56.
 XU Jia,WU Chun-lian,PENG Cong,et al.Role of Celastrol in Regulating Proliferation and Apoptosis of Lung Cancer Cell[J].Journal of China West Normal University(Natural Sciences),2014,35(01):51-56.
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雷公藤红素对人肺癌细胞增殖与凋亡的影响
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《西华师范大学学报(自然科学版)》[ISSN:1673-5072/CN:51-1699/N]

卷:
35
期数:
2014年01期
页码:
51-56
栏目:
出版日期:
2014-03-20

文章信息/Info

Title:
Role of Celastrol in Regulating Proliferation and Apoptosis of Lung Cancer Cell
作者:
徐佳伍春莲彭聪周海杨思杰
西华师范大学生命科学学院,四川 南充637009
Author(s):
XU JiaWU Chun-lianPENG CongZHOU HaiYANG Si-jie
College of Life Science,China West Normal University,Nanchong,637009,China
关键词:
雷公藤红素人肺癌细胞细胞活性细胞凋亡
Keywords:
lung cancercelastrol cellcell apoptosisDAPI
分类号:
R285
文献标志码:
b
摘要:
雷公藤红素是从传统中药雷公藤中提取分离得到的一种醌甲基三萜,具有抗炎、免疫抑制及抗肿瘤等药理活性.本实验研究雷公藤红素对肺癌细胞的增殖和凋亡的影响.用MTT法测定不同浓度雷公藤红素对肺癌细胞增殖的影响;用流式细胞仪检测肺癌细胞的凋亡率;用DAPI染色测定雷公藤红素对肺癌细胞的细胞核的影响.实验显示,雷公藤红素以时间和剂量依赖性的方式抑制肺癌细胞的增殖,并且晚期凋亡细胞和死亡细胞增多,细胞核逐渐破裂.实验结果表明,雷公藤红素具有抗肺癌的作用.
Abstract:
Extracted from traditional Chinese medicine tripterygium wilfordii,Celastrol is a quinone methyl terpene with antiinflammation,immune suppression and pharmacological activities of antitumor.The effects of Celastrol on proliferation and apoptosis of lung cancer cells were investigated in this experment.The proliferation inhibition of lung cancer cells induced by celastrol was measured by MTT;apoptosis rate of lung cancer cells was analyzed by flow cytometry;and the cell nucleus of lung cancer cells induced by celastrol was measured by DAPI fluorescence stain.Experimental results show that Celastrol induced time and dosedependent growth arrest of lung cancer cells and the number of the late apoptosis of cells and cell death increased.Moreover,the nucleus were gradually broken.Our results confirmed that Celastrol induces time and dose-dependent growth arrest of lung cancer cells and promotes the apoptosis.

备注/Memo

备注/Memo:
收稿日期:2013-10-21 基金项目:四川省教育厅重大培育项目(13CZ0029),三峡库区生态环境与生物资源省部共建重点实验室开放课题基金项目(SKL-2011-05). 作者简介:徐佳(1989-) ,女,宁夏石嘴山市人,西华师范大学生命科学学院硕士研究生,主要从事细胞学与天然抗肿瘤活性成分的研究. 通讯作者:伍春莲(1976-),女,四川彭州人,西华师范大学生命科学学院副教授,博士,主要从事细胞和分子生物学的研究和教学.
更新日期/Last Update: 2014-03-20