[1]廖咏梅,王佳婧.纤维素分解菌的分离与筛选[J].西华师范大学学报(自然科学版),2013,34(03):241-245.[doi:10.16246/j.issn.1673-5072.2013.03.020]
 LIAO Yong-mei,WANG Jia-jin.Isolation and Screening of Cellulose-decomposing Microorganisms[J].Journal of China West Normal University(Natural Sciences),2013,34(03):241-245.[doi:10.16246/j.issn.1673-5072.2013.03.020]
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纤维素分解菌的分离与筛选()
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《西华师范大学学报(自然科学版)》[ISSN:1673-5072/CN:51-1699/N]

卷:
34
期数:
2013年03期
页码:
241-245
栏目:
出版日期:
2013-09-20

文章信息/Info

Title:
Isolation and Screening of Cellulose-decomposing Microorganisms
作者:
廖咏梅;王佳婧;
西华师范大学生命科学学院;
Author(s):
LIAO Yong-meiWANG Jia-jin
( College of Life Science,China West Normal University,Nanchong 637000,China)
关键词:
滤纸酶活力纤维素降解菌筛选
Keywords:
filter paper enzyme activity cellulose-degrading microorganism screening
分类号:
Q93-3
DOI:
10.16246/j.issn.1673-5072.2013.03.020
文献标志码:
A
摘要:
以纤维素粉或滤纸条为唯一碳源,从海南土样中初筛出能够分解纤维素的菌株共6株,采用滤纸条液体培养基,纤维素固体培养基,刚果红鉴别培养基进行复筛,获取滤纸条断裂较明显,透明圈较大,红色水解圈较大的菌株2株,编号为Q-4,Q-6.通过对不同时间Q-4,Q-6 FPA酶活力的测定,确定48 h为其最佳发酵时间,而对不同接种量Q-4,Q-6 FPA酶活力的测定,确定两株菌的最佳接种量为5%.
Abstract:
6 strains of cellulose-decomposing microorganisms were isolated from the soil which were brought fromHaina province . Then they were screened by liquid culture of filtrates, solid culture of cellulose powder,congo redagarmedium with cellulose powder or filter paper as the sole carbon source. At last Q-4 and Q-6 strains were foundby degree of filter paper breakup and size of clarity circles . Afterward Q-4 and Q-6 strains were inoculated into liquidculture and the FPA ( Filter Paper Activity) was studied with different conditions.

参考文献/References:


[1] FAN L. T. ET A L. Cellulose Hydrolysis. New York: Spring-verlag, 1987: 121 - 147
[2] 陈敏. 一种改进的纤维素分解菌鉴别培养基. [J]杭州师范学院学报( 自然科学版) , 2001, 18( 5) :
[3] 叶姜瑜. 一株纤维素分解菌鉴别培养基.[J]微生物学通报, 1997, 24( 4)
[4] 中山大学生物系生物化学微生物学教研室编[M]. 生物化学技术导论. 北京: 人民教育出版社, 1981: 24 - 25
[5] 齐云. 一株能分解纤维素的高温耐碱放线菌[J]. 应用与环境生物学报. 2003. 9( 3)

备注/Memo

备注/Memo:

收稿日期: 2012 - 10 - 17
基金项目: 西南野生动植物资源保护教育部重点实验室开放基金XNYB11 - 1
作者简介: 廖咏梅( 1970 - ) ,女,四川达州人,西华师范大学生命科学学院副教授,主要从事环境生态学研究.
更新日期/Last Update: 2017-12-28