[1]王娅,蒋晓慧,周丽梅,等.一种双子表面活性剂与BSA相互作用的荧光研究[J].西华师范大学学报(自然科学版),2013,34(02):153-160.[doi:10.16246/j.issn.1673-5072.2013.02.006]
 WANG Ya,JIANG Xiao-hui,ZHOU Li-mei,et al.Fluorescence Study of A Gemini Surfactant Glycol bis-N-tetradecylNicotinate Bibromide with Bovine Serum Albumin[J].Journal of China West Normal University(Natural Sciences),2013,34(02):153-160.[doi:10.16246/j.issn.1673-5072.2013.02.006]
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一种双子表面活性剂与BSA相互作用的荧光研究()
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《西华师范大学学报(自然科学版)》[ISSN:1673-5072/CN:51-1699/N]

卷:
34
期数:
2013年02期
页码:
153-160
栏目:
出版日期:
2013-06-20

文章信息/Info

Title:
Fluorescence Study of A Gemini Surfactant Glycol bis-N-tetradecylNicotinate Bibromide with Bovine Serum Albumin
作者:
王娅;蒋晓慧;周丽梅;张瑜瑜;吴刚;王玉军;
化学合成与污染控制四川省重点实验室,西华师范大学化学化工学院;
Author(s):
WANG YaJIANG Xiao-huiZHOU Li-meiZHANG Yu-yuWU GangWANG Yu-jun
( Laboratory of Applied Chemistry and Pollution Control Technology College of Chemistry and Chemical Engineering,China West Normal University,Sichuan Nanchong 637009)
关键词:
双子表面活性剂牛血清白蛋白荧光光谱相互作用
Keywords:
Gemini surfactant bovine serum albumin fluorescence spectra interaction
分类号:
TS201.2;O657.3
DOI:
10.16246/j.issn.1673-5072.2013.02.006
文献标志码:
A
摘要:
采用荧光光谱法研究了双子表面活性剂溴化双-N-十四烷基二甘醇酯与牛血清白蛋白(BSA)的相互作用.实验结果表明该双子表面活性剂主要以静态猝灭和动态猝灭结合的方式猝灭BSA的内源荧光.计算了结合常数K和结合位点数n及热力学参数,结果表明两者的结合是一个吸热熵驱动的自发过程,疏水作用力是主要作用,但考虑到该双子表面活性剂的结构和所带的正电荷,及该条件下BSA中一些含芳香环的氨基酸残基及某些残基可能带负电荷,因此两者之间的静电相互作用和π-π堆积作用也不可忽略.竞争实验表明,表面活性剂的疏水链通过疏水作用插入到BSA的疏水微区,在位点I结合,从而引起蛋白质的构象变化.
Abstract:
The interaction between Gemini surfactant glycol bis-N-tetradecyl nicotinate dibromide with bovine serumalbumin ( BSA) was studied by fluorescence spectra. The results showed that glycol bis-N-tetradecyl nicotinate dibromidequenched the intrinsic fluorescence of BSA through a combined ( dynamic and static) procedure. Thebinding constant ( K) ,binding site ( n) and the thermodynamic parameters were calculated,and the data revealedthat the binding was a spontaneous,endothermic and entropy driving process and hydrophobic force predominatedthe interaction of glycol bis-N-tetradecyl nicotinate dibromide and BSA. However,the electrostatic interaction andπ-π stacking should be taken into account. Hydrophobic tails of glycol bis-N-tetradecyl nicotinate dibromide embeddedto the hydrophobic pocket of BSA in site I and altered the structure of BSA.

参考文献/References:


[1] 尹爱萍,白佛,李慧卿. 甲基紫与牛血清白蛋白作用的荧光光谱研究[J]. 分析试验室,2012,2( 31) : 71 - 74.
[2] WU D,XU G,FENG Y,et al. Comparative Study on Interaction of Bovine Serum Albumin with Dissymmetric and SymmetricGemini Surfactant by Spectral Method [J]. Colloid Polym Sci,2009,287: 225 - 230.
[3] KHAN A B,KHAN J M,ALI M S,et al. Interaction of Amphiphilic Drugs with Human and Bovine Serum Albumins[J].Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2012,97: 119 - 124.
[4] MIR M A,GULL N,KHAN J M,et al. Interaction of Bovine Serum Albumin with Cationic Single Chain + Nonionic and CationicGemini + Nonionic Binary Surfactant Mixtures [J]. J. Phys. Chem. B,2010,114: 3197 - 3204.
[5] 郭琼,李连之,董建方,刘鸿雁,薛泽春,许涛. 氧钒配合物[VO( o - Van - Asn) ( Phen) ]·1. 5CH3OH 的合成、晶体结构及与DNA 和BSA 的相互作用[J]. 化学学报, 2012,70: 1617 - 1624.
[6] GAO J Q,GUO Y W,WANG J,et al. Spectroscopic Analyses on Interaction of o-Vanillin-d-Phenylalanine,o-Vanillin-l-Tyrosineand o-Vanillin-l-Levodopa Schiff Bases with Bovine Serum Albumin ( BSA) [J]. Spectrochimica Acta Part A,2011,78:1278 - 1286.
[7] CHENG Z J. Interaction of Tetramethylpyrazine with Two Serum Albumins by A Hybrid Spectroscopic Method [J]. SpectrochimicaActa Part A,2012,93: 321 - 330.
[8] WU T Q,WU Q,GUAN S Y,et al. Binding of the Environmental Pollutant Naphthol to Bovine Serum Albumin [J]. Biomacromolecules,2007,8: 1899 - 1906.
[9] SAMANTA A,PAUL B K,GUCHHAIT N. Spectroscopic Probe Analysis for Exploring Probe-protein Interaction: A Mapping ofNative,Unfolding and Refolding of Protein Bovine Serum Albumin by Extrinsic Fluorescence Probe [J]. Biophysical Chemistry,2011,156: 128 - 139.
[10] 尚永辉,李华,孙家娟,刘彬,郑敏燕. 白杨素磺酸盐与牛血清白蛋白的相互作用[J]. 理化检测- 化学分册,2012,48: 155 - 161.
[11] 刘红星. 一种烟酸酯类季铵盐型Gemini 表面活性剂的合成与性能表征[M]. 西华师范大学硕士学位论文,2008,12 -18.
[12] LIU R H,YU X Y,GAO W,et al. Study on The Interaction Between Salvianic Acid A Sodium and Bovine Serum Albumin bySpectroscopic Methods [J]. Spectrochimica Acta Part A,2011,78: 1535 - 1539.
[13] LU S Y,YU X Y,YANG Y,et al. Spectroscopic Investigation on The Intermolecular Interaction Between N-confused porphyrins-(3-methylisoxazole) diad and Bovine Serum Albumin [J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2012,99: 116 - 121.
[14] LIU B S,WANG J,XUE C L,et al. Effects of Synthetic Food Colorants on The Interaction Between Norfloxacin and BovineSerum Albumin by Fluorescence Spectroscopy [J]. Monatsh Chem,2012,143: 401 - 408.
[15] WEI Y l,LI J Q,DONG C,et al. Investigation of The Association Behaviors Between Biliverdin and Bovine Serum Albuminby Fluorescence Spectroscopy [J]. Talanta,2006,70: 377 - 382.
[16] FENG X Z,LIN Z,YANG L J,et al. Investigation of The Interaction Between Acridine Orange and Bovine Serum Albumin[J]. Talanta,1998,47: 1223 - 1229.
[17] LI Q Y,ZHU Q C,DENG X Q,et al. Binding Interactions of Water-soluble Camptothecin Derivatives with Bovine Serum Albumin[J]. Spectrochimica Acta Part A,2012,86: 124 - 130.
[18] JANA S,DALAPAATI S,GHOSH S,et al. Binding Interaction Between Plasma Protein Bovine Serum Albumin and FlexibleCharge Transfer Fluorophore: A Spectroscopic Study in Combination with Molecular Docking and Molecular Dynamics Simulation[J]. Journal of Photochemistry and Photobiology A: Chemistry,2012,231: 19 - 27.
[19] WANG F,HUANG W,DAI Z X. Spectroscopic Investigation of The Interaction Between Riboflavin and Bovine Serum Albumin[J]. Journal of Molecular Structure,2008,875: 509 - 514.
[20] WANG Y X,LI L,SHENG L J,et al. Spectroscopic Study on The Inherent Binding Information of Cationic PerfluorinatedSurfactant with Bovine Serum Albumin [J]. Journal of Fluorine Chemistry,2011,132: 489 - 494.
[21] TIAN F F,JIANG F L,HAN X L,et al. Synthesis of A Novel Hydrazone Derivative and Biophysical Studies of Its Interactionswith Bovine Serum Albumin by Spectroscopic,Electrochemical,and Molecular Docking Methods [J]. J. Phys. Chem. B,2010,114: 14842 - 14853.
[22] HAN X L,MEI P,LIU Y,et al. Binding Interaction of Quinclorac with Bovine Serum Albumin: A Biophysical Study [J].Spectrochimica Acta Part A,2009,74: 781 - 787.
[23] CUI F L,WANG J L,CUI Y R,et al. Fluorescent Investigation of The Interactions Between N-( p-chlorophenyl) -N’-( 1-naphthyl) thiourea and Serum Albumin: Synchronous Fluorescence Determination of Serum Albumin [J]. Anal. Chim. Acta,2006,571: 175 - 183.
[24] HU Y J,YUE H L,LI X L,et al. Molecular Spectroscopic Studies on The Interaction of Morin with Bovine Serum Albumin[J]. Journal of Photochemistry and Photobiology B: Biology,2012,112: 16 - 22.
[25] WANG H C,JIANG X H,ZHOU L M,et al. Interaction of NAEn-s-n Gemini Surfactants with Bovine Serum Albumin: Astructure - Activity Probe [J]. J. Lumin,2013,134: 138 - 147.

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备注/Memo

备注/Memo:

收稿日期: 2012 - 01 - 13
基金项目: 四川省科技厅项目( 2009JY0062)
作者简介: 王娅( 1987 - ) ,女,四川巴中人,西华师范大学化学化工学院硕士研究生,主要从事精细有机合成研究.
通讯作者: 蒋晓慧( 1958 - ) ,女,四川蓬溪人,西华师范大学化学化工学院教授,主要从事精细化学品和有机化学的教学与研究.
更新日期/Last Update: 2017-12-28